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The copper binding occurs through a square-planar pyramid configuration involving nitrogen from histidines imidazole side chain, nitrogen from glycines alpha-amino group, deprotonated amide nitrogen of the glycine-histidine peptide bond, and carboxyl oxygen from lysine

In addition, HA and FLAG expression tags were added to both 5 and 3 ends, respectively

Acknowledgements The authors acknowledge financial support from the National Institutes of Health (NIH

5-Amino-1MQ 5 mg is supplied as a high-purity research compound, designed to provide consistent and reliable performance across experimental models investigating NNMT-related pathways